3K8B
Crystal structure of Turkey (Meleagiris gallopova)hemoglobin at 2.3 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.999, 79.515, 64.385 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 25.560 - 2.300 |
| R-factor | 0.26676 |
| Rwork | 0.264 |
| R-free | 0.31146 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1faw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.525 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 22860 | |
| <I/σ(I)> | 3 | 0.9 |
| Completeness [%] | 89.6 | 94.7 |
| Redundancy | 2.47 | 2.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | phosphate buffer pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
