3K8A
Neisseria gonorrhoeae PriB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-12-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | I 41 |
| Unit cell lengths | 74.240, 74.240, 140.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.250 - 2.700 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v1q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.440 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.072 | 0.220 |
| Number of reflections | 9741 | |
| <I/σ(I)> | 27 | 5.5 |
| Completeness [%] | 92.8 | 65.2 |
| Redundancy | 13.3 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 4% (w/v) PEG 4000, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
