3K81
Structure of the central interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-04-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.030, 68.605, 93.335 |
| Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
| Resolution | 33.467 - 3.400 |
| R-factor | 0.2693 |
| Rwork | 0.267 |
| R-free | 0.31980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k7u |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.544 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.112 | 0.338 |
| Number of reflections | 6402 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 92.3 | 61.2 |
| Redundancy | 3.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 15% PEG3350, 0.1M Citrate pH.4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
