3K7U
Structure of essential protein from Trypanosoma brucei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2009-06-25 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.362, 50.359, 44.150 |
Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
Resolution | 29.690 - 2.100 |
R-factor | 0.20538 |
Rwork | 0.202 |
R-free | 0.26459 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k80 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.842 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0095) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.060 |
High resolution limit [Å] | 2.100 | 1.990 |
Number of reflections | 15229 | |
<I/σ(I)> | 30.6 | 3.1 |
Completeness [%] | 96.4 | 76.2 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% PEG2000, 0.1 M Bis-tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |