3K71
Structure of integrin alphaX beta2 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 149.664, 165.745, 537.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.675 - 3.950 |
| R-factor | 0.3502 |
| Rwork | 0.350 |
| R-free | 0.37270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k6s |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.643 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.675 | 4.110 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.141 | 1.432 |
| Number of reflections | 117330 | |
| <I/σ(I)> | 12.6 | 1.9 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.55M tri-sodium citrate, 0.05M MgCl2, 0.1M imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
