3K6V
M. acetivorans Molybdate-Binding Protein (ModA) in Citrate-Bound Open Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 61 |
| Unit cell lengths | 81.629, 81.629, 104.636 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.620 - 1.690 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.975 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.620 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.054 | 0.593 |
| Number of reflections | 84835 | |
| <I/σ(I)> | 16.3 | 2.26 |
| Completeness [%] | 96.9 | 82.3 |
| Redundancy | 5.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 4mM Sodium Sulfate, 1.4M Ammonium Citrate, pH6.0, vapor diffusion, sitting drop, temperature 293K |
