3K6T

Crystal structure of the GLD-1 homodimerization domain from Caenorhabditis elegans at 2.04 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Detector technology	CCD
Collection date	2009-05-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	33.889, 42.962, 154.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.554 - 2.040
R-factor	0.201
Rwork	0.199
R-free	0.24500
Structure solution method	MAD + molecular replacement (native crystal)
RMSD bond length	0.005
RMSD bond angle	0.751
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	2.040	2.040
Rmerge	0.048	0.342
Number of reflections	15152
<I/σ(I)>	16.9	5.7
Completeness [%]	99.9	100
Redundancy	7.4	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	295	Sodium phosphate, citrate, LiSO4, isopropanol, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	4.2	295	Sodium phosphate, citrate, LiSO4, isopropanol, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K