3K6I
Crystal structure of chicken T-cadherin EC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.153, 44.930, 56.666 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.700 - 1.130 |
| R-factor | 0.143 |
| Rwork | 0.142 |
| R-free | 0.15800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k6f |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.041 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.150 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.117 | 0.186 |
| Number of reflections | 42837 | |
| <I/σ(I)> | 11.7 | 6.4 |
| Completeness [%] | 99.8 | 97.3 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 50mM Zn acetate, 20 % PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
