3K69
CRYSTAL STRUCTURE OF A PUTATIVE TRANSCRIPTIONAL REGULATOR (LP_0360) FROM LACTOBACILLUS PLANTARUM AT 1.95 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-09 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97959,0.97886 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.173, 38.054, 42.275 |
| Unit cell angles | 90.00, 110.73, 90.00 |
Refinement procedure
| Resolution | 28.307 - 1.950 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.393 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.307 | 28.310 | 2.000 |
| High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
| Rmerge | 0.093 | 0.031 | 0.691 |
| Total number of observations | 508 | 2023 | |
| Number of reflections | 12340 | ||
| <I/σ(I)> | 10.9 | 20.3 | 1 |
| Completeness [%] | 97.8 | 98 | 80.2 |
| Redundancy | 3.6 | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.2000M Na2HPO4, 20.0000% PEG-3350, No Buffer pH 4.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
