3K5Y

Crystal structure of FBF-2/gld-1 FBEa complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	97.226, 97.226, 101.275
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.613 - 2.300
Rwork	0.171
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k5q
RMSD bond length	0.006
RMSD bond angle	0.668
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.613	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	24164
<I/σ(I)>	22	4.4
Completeness [%]	100.0	100
Redundancy	11.5	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris pH 7.5, 10% polyethylene glycol 8000, and 8% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K