3K5S
Crystal structure of chicken T-cadherin EC1 EC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.804, 79.188, 105.162 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.000 - 2.900 |
Rwork | 0.213 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.121 | 0.305 |
Number of reflections | 10854 | |
<I/σ(I)> | 2.3 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 20% MPD, 15% PEG 400, 50mM Na acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |