3K5Q

Crystal structure of FBF-2/FBE complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97937
Spacegroup name	P 61
Unit cell lengths	98.803, 98.803, 103.039
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.857 - 2.200
Rwork	0.158
R-free	0.20260
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.678
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.857	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	28976
<I/σ(I)>	24.1	2.9
Completeness [%]	99.9	100
Redundancy	8.2	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	100 mM Bicine pH 9.0, 2% 1,4-Dioxane, 10% polyethylene glycol 20,000, and 10 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	293	100 mM Bicine pH 9.0, 2% 1,4-Dioxane, 10% polyethylene glycol 20,000, and 10 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K