3K5N

Crystal structure of E.coli Pol II-abasic DNA binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2007-12-27
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	88.412, 147.552, 96.279
Unit cell angles	90.00, 99.98, 90.00

Refinement procedure

Resolution	31.310 - 3.150
Rwork	0.232
R-free	0.23600
RMSD bond length	0.021
RMSD bond angle	2.327
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.150	3.150
Rmerge	0.131	0.569
Number of reflections	41921
<I/σ(I)>	11	2.6
Completeness [%]	99.7	99.6
Redundancy	4.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350, 0.3 M ammonium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K