3K5J

Crystal structure of Putative SUFU (suppressor of fused protein) homolog (YP_208451.1) from Neisseria gonorrhoeae FA 1090 at 1.40 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-08
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 32 2 1
Unit cell lengths	50.719, 50.719, 143.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.724 - 1.400
R-factor	0.136
Rwork	0.134
R-free	0.16200
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.604
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.724	28.724	1.450
High resolution limit [Å]	1.400	3.010	1.400
Rmerge	0.046	0.033	0.672
Number of reflections	43173	8210	8019
<I/σ(I)>	16.71	49.4	1.4
Completeness [%]	99.6	99.6	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	1.6000M (NH4)2SO4, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K