3K4U
CRYSTAL STRUCTURE OF putative binding component of ABC transporter from Wolinella succinogenes DSM 1740 complexed with lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-15 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.406, 149.151, 81.969 |
Unit cell angles | 90.00, 100.46, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.620 |
R-factor | 0.205 |
Rwork | 0.202 |
R-free | 0.26600 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.701 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.620 | 2.700 |
Rmerge | 0.124 | |
Number of reflections | 100715 | |
<I/σ(I)> | 7 | |
Completeness [%] | 99.3 | 92.3 |
Redundancy | 3.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 10% iso-propanol, 0.1 M Hepes, 20% PEG 4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |