3K4G
Crystal structure of E. coli RNA polymerase alpha subunit C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.342, 67.612, 116.553 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 47.023 - 2.050 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lb2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.775 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER (1.3.3) |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.036 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.082 | 0.544 |
| Total number of observations | 26693 | |
| Number of reflections | 50235 | |
| <I/σ(I)> | 12.9 | 2.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M hepes, 1.4M sodium citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
