3K4C
Pyranose 2-oxidase H167A/T169G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.03790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.206, 102.964, 137.122 |
| Unit cell angles | 90.00, 90.82, 90.00 |
Refinement procedure
| Resolution | 29.300 - 1.700 |
| R-factor | 0.18056 |
| Rwork | 0.180 |
| R-free | 0.21631 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1tt0 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.071 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.5) |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 295544 | |
| <I/σ(I)> | 12.3 | 1.8 |
| Completeness [%] | 96.8 | 98.3 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 0.1M MES, 50MM MGCL2, 10% (W/V)% MONOMETHYLETHER PEG 2000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
