3K44
Crystal Structure of Drosophila melanogaster Pur-alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-04 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 63.168, 62.544, 64.479 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 44.460 - 2.100 |
R-factor | 0.223 |
Rwork | 0.222 |
R-free | 0.24000 |
Structure solution method | MAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CRUNCH2 |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.479 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.442 | |
Number of reflections | 28601 | |
<I/σ(I)> | 4.35 | |
Completeness [%] | 98.9 | 94.6 |
Redundancy | 5.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM Mes, 200 mM MgCl2, 25% PEG 3350, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM Mes, 200 mM MgCl2, 25% PEG 3350, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |