3K42
Crystal structure of sCD-MPR mutant E19Q/K137M pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 |
| Unit cell lengths | 102.582, 102.582, 100.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.270 - 2.300 |
| Rwork | 0.228 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6p |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.093 | 0.481 |
| Number of reflections | 23014 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.1M HEPES pH 7.0, 3M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
