3K3V

Crystal structure the GYF domain of S. Cerevisiae SMY2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.91841
Spacegroup name	P 65 2 2
Unit cell lengths	68.400, 68.400, 111.321
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.190 - 1.800
Rwork	0.208
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fma
RMSD bond length	0.011
RMSD bond angle	1.497
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Refinement software	REFMAC (5.5.0063)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.190	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.075	0.656
Total number of observations		42500
Number of reflections	14884
<I/σ(I)>	31.4	5.2
Completeness [%]	99.8	99.7
Redundancy	20.9	20.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	18% polyacrylic acid, 0.15M MgCl2, 0.1M Na-HEPES, pH 7.5, Vapor diffusion, sitting drop, temperature 293K