3K2V
Structure of the CBS pair of a putative D-arabinose 5-phosphate isomerase from Klebsiella pneumoniae subsp. pneumoniae.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931, 0.97945 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 102.151, 102.151, 214.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.580 - 1.950 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.18200 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.532 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.108 | 0.676 |
| Number of reflections | 31947 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.2 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | 0.1M Tris pH8.5, 0.2M Ammonium Sulfate, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
