3K28
Crystal Structure of a glutamate-1-semialdehyde aminotransferase from Bacillus anthracis with bound Pyridoxal 5'Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-31 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.809, 113.688, 113.955 |
| Unit cell angles | 90.00, 118.65, 90.00 |
Refinement procedure
| Resolution | 28.450 - 1.950 |
| Rwork | 0.147 |
| R-free | 0.18540 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CRANK |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.103 | 0.568 |
| Number of reflections | 240968 | |
| <I/σ(I)> | 17.4 | 2.58 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 18% PEG8K, 0.2M caAcet., 0.1M Nacacod pH 6.5, 10mM PLP , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
