3K25
Crystal Structure of Slr1438 protein from Synechocystis sp. PCC 6803, Northeast Structural Genomics Consortium Target SgR112
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97926 |
Spacegroup name | P 65 |
Unit cell lengths | 145.566, 145.566, 60.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.648 - 2.551 |
R-factor | 0.2331 |
Rwork | 0.233 |
R-free | 0.24390 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.394 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.4_115) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.083 | 0.510 |
Number of reflections | 46233 | |
<I/σ(I)> | 21.1 | 3.3 |
Completeness [%] | 98.3 | |
Redundancy | 8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: PEG-8K 20%, Nh4H2Po4, n-Butanol 0.7%, 2,2,2-Trifluororthanol 4.0%, VAPOR DIFFUSION, HANGING DROP, temperature 293K |