3K21
Crystal Structure of carboxy-terminus of PFC0420w.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | .97920 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 50.469, 50.469, 108.656 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.730 - 1.150 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 2.404 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.170 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.078 | 0.547 |
Number of reflections | 56865 | |
<I/σ(I)> | 11.8 | 1.57 |
Completeness [%] | 98.2 | 77.6 |
Redundancy | 9 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 22 % PEG 3350, 0.1 M NaAcet pH 4.2, 10 mM CaCl2, 2 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |