3K1L
Crystal Structure of FANCL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0397 |
Spacegroup name | H 3 2 |
Unit cell lengths | 188.680, 188.680, 259.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.926 - 3.200 |
R-factor | 0.2072 |
Rwork | 0.205 |
R-free | 0.24660 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.126 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.926 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.089 | 0.479 |
Number of reflections | 25772 | |
<I/σ(I)> | 6.3 | 5.2 |
Completeness [%] | 98.8 | 100 |
Redundancy | 12.2 | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1M Ammonium Citrate, 100mM Bis-Tris-Propane, 1mM TCEP, 0.050mM ZNCl2, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |