3K1K
Green fluorescent protein bound to enhancer nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77.2 |
| Detector technology | PIXEL |
| Collection date | 2008-07-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 160.470, 160.470, 78.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.048 - 2.150 |
| R-factor | 0.2146 |
| Rwork | 0.212 |
| R-free | 0.25470 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.048 | 2.290 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 56271 | |
| <I/σ(I)> | 14.95 | 2.25 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 3.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 60% MPD, 100MM NAAC PH 4.6, 10MM CACL2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
