3K1K
Green fluorescent protein bound to enhancer nanobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 77.2 |
Detector technology | PIXEL |
Collection date | 2008-07-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 160.470, 160.470, 78.828 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.048 - 2.150 |
R-factor | 0.2146 |
Rwork | 0.212 |
R-free | 0.25470 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.048 | 2.290 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 56271 | |
<I/σ(I)> | 14.95 | 2.25 |
Completeness [%] | 99.3 | 98.9 |
Redundancy | 3.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 60% MPD, 100MM NAAC PH 4.6, 10MM CACL2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |