3K1I
Crystal strcture of FliS-HP1076 complex in H. pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 41 |
| Unit cell lengths | 50.253, 50.253, 242.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.330 - 2.700 |
| R-factor | 0.26024 |
| Rwork | 0.258 |
| R-free | 0.29869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.442 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | 0.659 |
| Number of reflections | 17571 | |
| <I/σ(I)> | 20.6 | 2.05 |
| Completeness [%] | 94.2 | 81.4 |
| Redundancy | 4.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 0.1M MES, 0.2M Calcium acetate, 3%(v/v) 2-propanol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
