3K1D
Crystal structure of glycogen branching enzyme synonym: 1,4-alpha-D-glucan:1,4-alpha-D-GLUCAN 6-glucosyl-transferase from mycobacterium tuberculosis H37RV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9897 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 109.373, 156.861, 48.021 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.330 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB CODE 1M7X |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.410 |
High resolution limit [Å] | 2.330 | 2.330 |
Number of reflections | 36272 | |
<I/σ(I)> | 12.9 | 2.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.4 | 15.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 297K |