3K17

Crystal structure of a Lin0012 protein from Listeria innocua

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	105.966, 122.362, 138.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.060 - 2.100
R-factor	0.223
Rwork	0.223
R-free	0.25200
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.060	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.118	0.585
Number of reflections	105467
<I/σ(I)>	10.7	2.5
Completeness [%]	100.0	100
Redundancy	15.8	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.2M MgCl2.6H2o, 0.1M HEPES, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K