3K0W

Crystal structure of the tandem IG-like C2-type 2 domains of the human mucosa-associated lymphoid tissue lymphoma translocation protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97920
Spacegroup name	P 62 2 2
Unit cell lengths	90.355, 90.355, 182.173
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.470 - 2.800
R-factor	0.2196
Rwork	0.218
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bfo
RMSD bond length	0.010
RMSD bond angle	1.320
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.470	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	11498
<I/σ(I)>	43.44	2.82
Completeness [%]	99.9	91.8
Redundancy	18.8	17.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	289	1:1 VOLUME RATIO OF PROTEIN SOLUTION (10 mg/mL) AND RESERVOIR SOLUTION (1.5 M MAGNESIUM CHLORIDE, 0.1 M BICINE pH 9.0), VAPOR DIFFUSION, SITTING DROP, temperature 289K