3K0B
Crystal structure of a predicted N6-adenine-specific DNA methylase from Listeria monocytogenes str. 4b F2365
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.949, 46.156, 91.376 |
Unit cell angles | 90.00, 131.09, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.500 |
R-factor | 0.16591 |
Rwork | 0.164 |
R-free | 0.19771 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.625 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.058 | 0.520 |
Number of reflections | 62193 | |
<I/σ(I)> | 32 | 2.1 |
Completeness [%] | 98.0 | 95.6 |
Redundancy | 3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M Ammonium sulfate 25 % PEG3350 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |