3K07
Crystal structure of CusA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 178.393, 178.393, 285.761 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.285 - 3.521 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.27900 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.874 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.650 |
| High resolution limit [Å] | 3.520 | 3.520 |
| Rmerge | 0.057 | 0.489 |
| Number of reflections | 21591 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 3350, 0.1M Na citrate, 0.5M (NH4)2SO4, 10% Glycerol, 1% JM600 pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
