3JZZ

Crystal structure of Pseudomonas aeruginosa (strain: Pa110594) typeIV pilin in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-13
Detector	ADSC QUANTUM 210
Wavelength(s)	1.10000
Spacegroup name	P 21 21 21
Unit cell lengths	29.460, 44.869, 88.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.966 - 1.597
R-factor	0.1842
Rwork	0.180
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jyz
RMSD bond length	0.023
RMSD bond angle	1.891
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.597
Rmerge	0.058
Number of reflections	16182
<I/σ(I)>	31.5
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	0.1 M Hepes pH 7.5, 20% w/v PEG 1000, 8% v/v Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 294K