3JZV
Crystal structure of Rru_A2000 from Rhodospirillum rubrum: A cupin-2 domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | H 3 2 |
Unit cell lengths | 90.886, 90.886, 161.252 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.580 - 2.300 |
R-factor | 0.227 |
Rwork | 0.225 |
R-free | 0.27800 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.492 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.210 | 2.300 |
Rmerge | 0.106 | 0.097 | 0.416 |
Number of reflections | 11778 | ||
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.9 | 98 | 100 |
Redundancy | 7 | 6.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 15 % MPD, 0.1M Sodium Hepes pH 7.5, 0.8M Potassium Sodium Tartrate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 15 % MPD, 0.1M Sodium Hepes pH 7.5, 0.8M Potassium Sodium Tartrate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |