3JZP
Human MDMX liganded with a 12mer peptide inhibitor (pDI6W)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.369, 27.632, 52.135 |
Unit cell angles | 90.00, 124.57, 90.00 |
Refinement procedure
Resolution | 28.970 - 1.740 |
R-factor | 0.16 |
Rwork | 0.160 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dab |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.790 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.049 | 0.091 |
Number of reflections | 8289 | |
<I/σ(I)> | 21.2 | 15.1 |
Completeness [%] | 93.9 | 77 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 292 | 1.4 M Na/K phosphate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |