3JZM
Crystal structure of the phosphorylation-site mutant T432A of the KaiC circadian clock protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 113.15 |
| Detector technology | CCD |
| Collection date | 2008-02-24 |
| Detector | MAR 300mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.370, 135.110, 204.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.240 - 2.900 |
| Rwork | 0.228 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbl |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.240 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.072 | 0.564 |
| Number of reflections | 77061 | |
| <I/σ(I)> | 17.8 | 2.8 |
| Completeness [%] | 93.8 | 84.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | SODIUM FORMATE, GLYCEROL, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
