3JZ4

Crystal structure of E. coli NADP dependent enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95364
Spacegroup name	P 4 21 2
Unit cell lengths	151.885, 151.885, 165.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.560 - 2.300
R-factor	0.17
Rwork	0.168
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.297
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.560
High resolution limit [Å]	2.300	2.300
Rmerge	0.188	0.188
Number of reflections	74567
<I/σ(I)>		3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.2 M ammonium tartrate, 30 % PEG 3350, 10 mM 2-mercaptoethanol, 0.1 M tris, pH 7.5, temperature 298K, VAPOR DIFFUSION, HANGING DROP