3JXU
Crystal structure of the human 70kDa heat shock protein 1A (Hsp70-1) ATPase domain in complex with ADP and inorganic phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Detector technology | CCD |
| Collection date | 2008-09-26 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54166 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.013, 63.298, 144.197 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.540 - 2.140 |
| R-factor | 0.20806 |
| Rwork | 0.205 |
| R-free | 0.26181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ba0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.407 |
| Data reduction software | SAINT |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.430 |
| High resolution limit [Å] | 2.140 |
| Rmerge | 0.066 |
| Number of reflections | 23808 |
| Completeness [%] | 98.3 |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.2M magnesium chloride hexahydrate, 0.1M bis-tris, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
