3JXJ

Crystal structure of the chicken TRPV4 ankyrin repeat domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2006-02-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	47.771, 77.333, 186.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.770 - 2.800
R-factor	0.264
Rwork	0.262
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRPV4-ARD (3jxi)
RMSD bond length	0.012
RMSD bond angle	1.079
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.135	0.078	0.172
Number of reflections	14247
<I/σ(I)>	6.8
Completeness [%]	80.6	99.7	30.1
Redundancy	6.1	8.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.025 M KH2PO4, 7% MPD, 7% PEG8000, and 5 mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K