3JXF
CA-like domain of human PTPRZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-11 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.963, 68.118, 65.682 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 35.943 - 2.004 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.969 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.115 | 0.072 | 0.381 |
| Number of reflections | 37363 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 99.9 | 99.7 | 99.3 |
| Redundancy | 3.6 | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM Na-cacodylate pH 6.5, 200 mM Magnesium acetate, 20% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
