3JXF
CA-like domain of human PTPRZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-03-11 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.963, 68.118, 65.682 |
Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
Resolution | 35.943 - 2.004 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.969 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.115 | 0.072 | 0.381 |
Number of reflections | 37363 | ||
<I/σ(I)> | 11.5 | ||
Completeness [%] | 99.9 | 99.7 | 99.3 |
Redundancy | 3.6 | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM Na-cacodylate pH 6.5, 200 mM Magnesium acetate, 20% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |