3JXD
Crystal structure of the P22 c2 repressor protein in complex with synthetic operator 9C in the presence of Rb+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-03-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 |
Unit cell lengths | 64.007, 64.007, 101.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.340 - 2.100 |
R-factor | 0.183 |
Rwork | 0.180 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r1j |
RMSD bond length | 0.020 |
RMSD bond angle | 2.229 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.340 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 23743 | |
<I/σ(I)> | 33.2 | 4.5 |
Completeness [%] | 99.3 | 99.6 |
Redundancy | 12.5 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | Rubidium chloride, PEG 400, Tris-HCl, MgCl2, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Rubidium chloride | ||
2 | 1 | 1 | PEG 400 | ||
3 | 1 | 1 | Tris-HCl | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 2 | Rubidium chloride | ||
6 | 1 | 2 | PEG 400 | ||
7 | 1 | 2 | Tris-HCl | ||
8 | 1 | 2 | MgCl2 |