3JXA

Immunoglobulin domains 1-4 of mouse CNTN4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-27
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	281.728, 40.431, 95.076
Unit cell angles	90.00, 103.32, 90.00

Refinement procedure

Resolution	37.172 - 2.403
R-factor	0.1997
Rwork	0.197
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.932
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.136	0.087	0.548
Number of reflections	40236
<I/σ(I)>	8.8
Completeness [%]	97.0	99.7	81.1
Redundancy	9.2	10.5	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	20 mM Na/K phosphate pH 5.8, 10% PEG 3400, 1.2 M 1,6-hexanediol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	20 mM Na/K phosphate pH 5.8, 10% PEG 3400, 1.2 M 1,6-hexanediol, VAPOR DIFFUSION, HANGING DROP, temperature 293K