3JWN
Complex of FimC, FimF, FimG and FimH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 216.025, 216.025, 532.072 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.340 - 2.690 |
| R-factor | 0.24506 |
| Rwork | 0.244 |
| R-free | 0.27208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1KLF 3BWU and 3BFQ |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0096) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 176.800 | 2.790 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.147 | 0.862 |
| Number of reflections | 119926 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 90.6 | 46.7 |
| Redundancy | 9.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 293 | 1.6 M potassium chloride, 0.1 M sodium citrate, pH 4.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
