3JVV
Crystal Structure of P. aeruginosa PilT with bound AMP-PCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.483, 119.552, 185.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.120 - 2.600 |
| R-factor | 0.24667 |
| Rwork | 0.244 |
| R-free | 0.29136 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.120 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.052 | 0.422 |
| Number of reflections | 35387 | |
| <I/σ(I)> | 32.6 | 2.8 |
| Completeness [%] | 94.4 | 93.6 |
| Redundancy | 7.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | mother liquor: 10% PEG 6000, 0.1 M HEPES, protein buffer contains NaCl, MES, glycerol, MgCl and citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
