3JVU
Crystal structure of unliganded P. aeruginosa PilT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-05 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.240, 121.378, 184.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.340 - 3.100 |
| R-factor | 0.23165 |
| Rwork | 0.229 |
| R-free | 0.28195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.477 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 20179 | |
| <I/σ(I)> | 17.3 | 3 |
| Completeness [%] | 94.9 | 76.1 |
| Redundancy | 7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Mother liquor: 100 mM Hepes, 8% PEG 6000, Protein buffer contains MES, NaCl, glycerol, citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
