3JVS
Characterization of the Chk1 allosteric inhibitor binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 103.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.754, 65.578, 57.931 |
| Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.187 |
| R-free | 0.21500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.787 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 26131 | |
| Completeness [%] | 98.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 283 | 2.5-8% (w/v) PEG 8K, 0.1 M Hepes, 32-36% (v/v) ethylene glycol in 3-5 days, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 283.0K |
