3JVL
Crystal structure of bromodomain 2 of mouse Brd4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97642 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 52.060, 73.050, 32.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.530 - 1.200 |
R-factor | 0.117 |
Rwork | 0.116 |
R-free | 0.14500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ouo |
RMSD bond length | 0.031 |
RMSD bond angle | 2.551 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.530 | 1.250 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.043 | 0.381 |
Number of reflections | 39272 | 4460 |
<I/σ(I)> | 20.37 | 4 |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris/HCl, 25% PEG 2000 MME, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |