3JVD

Crystal structure of putative transcription regulation repressor (LACI family) FROM Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	90
Detector technology	CCD
Collection date	2009-08-24
Detector	ADSC QUANTUM 315
Spacegroup name	P 65 2 2
Unit cell lengths	91.970, 91.970, 285.453
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.22604
Rwork	0.224
R-free	0.28353
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.130
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.3.0034)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge		0.950
Number of reflections	32657
<I/σ(I)>	5.1	1
Completeness [%]	100.0	100
Redundancy	11.7	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5		0.1M SODIUM CACODYLATE, PH 6.5, 2M AMMONIUM SULFATE, 200MM SODIUM CHLORIDE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K