3JVC
Crystal Structure of the Lipoprotein_17 domain from Q9PRA0_UREPA protein of Ureaplasma parvum. Northeast Structural Genomics Consortium Target UuR17a.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97914 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.560, 76.829, 214.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.420 - 2.690 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.790 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.061 | 0.212 |
| Number of reflections | 22095 | |
| <I/σ(I)> | 52.2 | 14.2 |
| Completeness [%] | 98.5 | 94 |
| Redundancy | 11.9 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under paraffin oil | 7.5 | 291 | 24% PEG 20000, 0.1M RbCl, 0.1M HEPES, pH 7.5, microbatch under paraffin oil, temperature 291K |
