3JV5

Crystal structure of the dimerization domains p52 homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	106
Detector technology	IMAGE PLATE
Collection date	2007-06-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	46.433, 56.863, 102.192
Unit cell angles	90.00, 97.77, 90.00

Refinement procedure

Resolution	19.710 - 2.650
Rwork	0.209
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.740
High resolution limit [Å]	2.650	2.650
Number of reflections	13577
<I/σ(I)>	10.1	2
Completeness [%]	88.0	64
Redundancy	6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	PEG8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K